Real-time PCR is currently used in many clinical microbiology laboratories to diagnose microbial infections. This method combines PCR chemistry with fluorescent probe detection of amplified product in the same reaction vessel. Real-time PCR assays can be completed in an hour or less and provide sensitivity and specificity. The combination of high sensitivity and specificity, low contamination risk, and speed has made real-time PCR technology a popular method for diagnosing many infectious diseases.

Further reading:

Real-Time PCR in Microbiology: From Diagnosis to Characterization Ian M. Mackay (2007)