from Patrizia Caposio, Daniel N. Streblow and Jay A. Nelson writing in Cytomegaloviruses: From Molecular Pathogenesis to Intervention:
Proteomics is the large-scale study of proteins, particularly their structure and interaction. In this review we will discuss the results obtained using a proteomic approach to analyse what is secreted from cytomegalovirus infected cells: the composition of the viral and subviral particles as well as the cellular factors that are involved in the viral pathogenesis. In the previous edition we described the viral and cellular proteins that compose the infectious HCMV virion, the entry competent, non-replicating viral particles such as Dense Bodies (DBs) and Non-Infectious Enveloped Particles (NIEPs). Using a gel-free 2-D capillary liquid chromatography (LC)-MS/MS and a Fourier transform ion cyclotron resonance (FTICR) mass spectrometry we were able to identify the relative abundance of viral and cellular proteins in purified HCMV particles. The first part of this review will be an up-date of the literature that has been published in these last few years on the structure and composition of the viral particles. The second part of the review will be dedicated to the analysis of the cellular factors secreted from infected cells that act in a paracrine fashion to enhance wound healing (WH) and angiogenesis (AG) associated with the development of long term diseases like atherosclerosis, transplant vascular sclerosis (TVS), chronic allograft rejection (CR) and glioblastoma.
Further reading: Cytomegaloviruses: From Molecular Pathogenesis to Intervention