HSV-1 Replication
HSV-1 DNA Replication
Category: Virology
from Stacey A. Leisenfelder and Sandra K. Weller writing in Alphaherpesviruses: Molecular Virology:
The cis- and trans-acting elements required for DNA synthesis of Herpes Simplex Virus (HSV) have been identified, and genetic and biochemical analyses have provided important insights into how they work together to replicate the large double-stranded viral genome. Furthermore, viral enzymes involved in DNA replication have provided a rich store of useful targets for antiviral therapy against herpesviruses. Despite these advances, many questions remain unresolved concerning the overall mechanism of genome replication. For instance, it has long been recognized that the products of viral DNA replication are head-to-tail concatemers; however, it is not clear how these concatemers are generated. A recent review summarizes the known functions of viral replication proteins and explore the possibility that these viral proteins may function in combination with cellular proteins to produce concatemers suitable for packaging into preformed viral capsids.
Further reading: Alphaherpesviruses: Molecular Virology
The cis- and trans-acting elements required for DNA synthesis of Herpes Simplex Virus (HSV) have been identified, and genetic and biochemical analyses have provided important insights into how they work together to replicate the large double-stranded viral genome. Furthermore, viral enzymes involved in DNA replication have provided a rich store of useful targets for antiviral therapy against herpesviruses. Despite these advances, many questions remain unresolved concerning the overall mechanism of genome replication. For instance, it has long been recognized that the products of viral DNA replication are head-to-tail concatemers; however, it is not clear how these concatemers are generated. A recent review summarizes the known functions of viral replication proteins and explore the possibility that these viral proteins may function in combination with cellular proteins to produce concatemers suitable for packaging into preformed viral capsids.
Further reading: Alphaherpesviruses: Molecular Virology
Roles of ICP22 in HSV-1 Replication
Category: Virology
from Stephen A. Rice writing in Alphaherpesviruses: Molecular Virology:
ICP22 is the least characterized of the five herpes simplex virus type 1 (HSV-1) immediate-early (IE) proteins. However, accumulating evidence indicates that it carries out a number of interesting regulatory activities inside the infected cell. These include the enhancement of viral gene expression, the modification of RNA polymerase II (RNAP II), and the reorganization of host cell molecular chaperones into nuclear inclusion bodies. Recent studies of engineered HSV-1 mutants indicate that certain of ICP22's activities are genetically separable from each other. Thus, similar to several other of the IE proteins, ICP22 appears to be a multifunctional, multi-domain polypeptide. A recent review summarizes the current state of knowledge concerning ICP22 and its varied regulatory roles during the productive HSV-1 infection.
Further reading: Alphaherpesviruses: Molecular Virology
ICP22 is the least characterized of the five herpes simplex virus type 1 (HSV-1) immediate-early (IE) proteins. However, accumulating evidence indicates that it carries out a number of interesting regulatory activities inside the infected cell. These include the enhancement of viral gene expression, the modification of RNA polymerase II (RNAP II), and the reorganization of host cell molecular chaperones into nuclear inclusion bodies. Recent studies of engineered HSV-1 mutants indicate that certain of ICP22's activities are genetically separable from each other. Thus, similar to several other of the IE proteins, ICP22 appears to be a multifunctional, multi-domain polypeptide. A recent review summarizes the current state of knowledge concerning ICP22 and its varied regulatory roles during the productive HSV-1 infection.
Further reading: Alphaherpesviruses: Molecular Virology