Rapid and accurate diagnostic methods for typing and subtyping influenza viruses are needed for improved worldwide surveillance. Although molecular-based diagnostic methods are becoming more widespread in influenza diagnosis, they generally involve amplification of the hemagglutinin (HA) and/or neuraminidase (NA) gene segments for subtyping. A low-density microarray (MChip) has been developed that allows for the identification and subtyping of influenza A viruses in approximately seven hours.

MChip is unique in that it is based solely on the matrix (M) gene segment which has enough genetic diversity for subtype analysis but sufficient genetic stability to circumvent the need for continual redesign of primers and microarray probes.

Further reading:

• Influenza: Molecular Virology

Labels: Influenza matrix, Influenza microarray, Matrix gene segment, MChip, microarray