Rapid and accurate diagnostic methods for typing and subtyping
influenza viruses are needed for improved worldwide surveillance. Although molecular-based diagnostic methods are becoming more widespread in
influenza diagnosis, they generally involve amplification of the hemagglutinin (HA) and/or neuraminidase (NA) gene segments for subtyping. A low-density microarray (MChip) has been developed that allows for the identification and subtyping of influenza A viruses in approximately seven hours.
MChip is unique in that it is based solely on the matrix (M) gene segment which has enough genetic diversity for subtype analysis but sufficient genetic stability to circumvent the need for continual redesign of primers and microarray probes.
Further reading:
Labels: Influenza matrix, Influenza microarray, Matrix gene segment, MChip, microarray
A
DNA microarray is a multiplex technology that can be used in microbiology to study gene expression of thousands of genes simultaneously, to analyze the genomes of different microorganisms and to identify or diagnose microorganisms, for example in food and feed, mycoplasms in cell culture, or pathogens for disease detection.
In standard microarrays a small piece of glass or silicon is used as the solid surface for the microarray assay. These are commonly known as gene chips, biochips or "
lab on a chip".
A new two-volume book "Lab-on-a-Chip Technology" was published recently. The book describes the recent innovations in the microarray field and the applications of microarray technology in the fields of microbiology, molecular biology, biotechnology and bioscience.
Lab-on-a-Chip Technology: Biomolecular Separation and Analysis ISBN: 978-1-904455-47-9
Lab-on-a-Chip Technology: Fabrication and Microfluidics ISBN: 978-1-904455-46-2
CURRENT BOOKS OF INTEREST
Metagenomics: Theory, Methods and ApplicationsAspergillus: Molecular Biology and GenomicsEnvironmental Molecular MicrobiologyNeisseria: Molecular Mechanisms of PathogenesisFrontiers in Dengue Virus ResearchABC Transporters in MicroorganismsPili and FlagellaLab-on-a-Chip Technology: Biomolecular Separation and AnalysisLab-on-a-Chip Technology: Fabrication and MicrofluidicsBacterial PolysaccharidesMicrobial ToxinsAcanthamoebaBacterial Secreted ProteinsLactobacillusMycobacteriumReal-Time PCRClostridiaPlant Pathogenic BacteriaBiopolymersPlasmidsPasteurellaceaeVibrio choleraePathogenic FungiHelicobacter pyloriCorynebacteriaStaphylococcusLeishmaniaArchaeaLegionellaRNA and the Regulation of Gene ExpressionMolecular Oral MicrobiologyLabels: biochip, biochips, lab on a chip, microarray, microarrays