Book review
Real-Time PCR in Microbiology: From Diagnosis to Characterization
Rapid molecular methods, like real-time PCR, provide new possibilities for the diagnosis and treatment of infectious diseases. This book gives us a glimpse into this cutting edge technology and the applications thereof in diagnostic laboratories, namely detection, quantification and characterization of microorganisms. The first part of the book is an introduction to real-time PCR, the different oligonucleotide (primer) and probe chemistries used; sample collection and preparation of target nucleic acids; and primer design. A comprehensive chapter on the use of standards and controls gives invaluable advice for diagnostic laboratories. Quantification of microorganisms is also discussed in depth. The latter part of the book involves the use of real-time PCR in routine diagnostics of bacteria, viruses and agents of bioterrorism. I particularly liked the mention of future trends and as well as references to web sites at the end of some chapters and also enjoyed reading the Round Table discussion where experts gave answers to the different questions asked. This up-to-date book on real-time PCR should be available on the bench in all diagnostic microbiology and virology laboratories. I wish I had access to this book when I started doing real-time PCR some years ago.
I really enjoyed reading it and can recommend it to anyone using the technique in a diagnostic laboratory.
Review by: Susan Engelbrecht, Division of Medical Virology, University of Stellenbosch and NHLS, Tygerberg, South Africa
Full details of the book available at Real-Time PCR in Microbiology: From Diagnosis to Characterization