Fluorescence
Fluorescence is the emission of electromagnetic radiation, especially of visible light, stimulated in a substance by the absorption of incident radiation and persisting only as long as the stimulating radiation is continued. Fluorescence is important in molecular biology. Fluorescence is used in DNA sequencing. Fluorescence is used in labeling and visualising DNA. Fluorescence is used in DNA chip technology, fluorescence microscopy and green fluorescent protein GFP. | Edited by: Martin Filion "useful book ... filled with valuable information" (Doodys) read more ...Aimed specifically at microbiologists, this volume describes and explains the most important aspects of current real-time quantitative PCR (qPCR) strategies, instrumentation and software. |
Fluorescence
Fluorescence is important in automated sequencing of DNA. Each of four different chain terminating bases has its own specific fluorescent tag. In fluorescent sequencing the labelled DNA molecules are separated, the fluorescent label is excited by a UV source, and the base is identified by the wavelength of the emitted light. Fluorescence is used in DNA detection. Ethidium bromide has low fluorescence until it binds to DNA. This fluorescence is ussful in visualising the location of DNA fragments in agarose gel electrophoresis. Fluorescence is used in DNA chip technology. Fluorescence is used in microscopy and immunology. FACS (fluorescent-activated cell sorting). Fluorescence can be useful in analysis of the structure and conformations of DNA and proteins. Aequorea victoria, produces a blue fluorescence in the presence of Ca2+ ions. Green Fluorescent Protein (GFP) is an important research tool. The fluorescence of GFP and related proteins are used as reporters in a number of systems. Gene expression can be measured by fluorescence by fusion of a GFP gene to the gene being studied.Molecular Biology Resources
Molecular Biology GatewayPCR Information and links on PCR
PCR Protocols
Real-Time PCR
The Genome Gateway
The Genomics Gateway
The Protocols Gateway
New Molecular Biology Sites
Recommended Reading
 | Edited by: Kevin V. Morris "an excellent resource" (Doodys); "a comprehensive review ... a must-have" (Epigenetics) read more ...An important and up-to-date overview of the modulation of gene transcription by non-coding RNAs. An essential reference book and a major information resource for those working in the area. |