Oligonucleotide microarrays
DNA oligonucleotides are spotted onto the array. The main feature of oligonucleotide microarrays is that each gene is normally represented by more than one probe. | Edited by: Martin Filion "useful book ... filled with valuable information" (Doodys) read more ...Aimed specifically at microbiologists, this volume describes and explains the most important aspects of current real-time quantitative PCR (qPCR) strategies, instrumentation and software. |
Oligonucleotide microarrays
In oligonucleotide microarrays, short DNA oligonucleotides are spotted onto the array. The main feature of oligonucleotide microarray is that each gene is normally represented by more than one probe: the ensemble of probes mapping to different regions of the gene is usually called probe set. Commercial oligonucleotide chips are available (e.g. Affymetrix, Inc. GeneChip system) with an oligonucleotide representation of tens of thousands genes, covering an entire genome; different genomes are currently available (human, mouse, Drosophila and others). For technical reasons, only a single sample can be measured on one of these chips; thus experiments using oligonucleotides microrarrays are often referred as single channel hybridization. This implies that to have data of differential expression between one sample (test sample) and another (reference sample), two separate hybridizations, one for each chip, have to be performed.Molecular Biology Resources
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Recommended Reading
 | Edited by: Kevin V. Morris "an excellent resource" (Doodys); "a comprehensive review ... a must-have" (Epigenetics) read more ...An important and up-to-date overview of the modulation of gene transcription by non-coding RNAs. An essential reference book and a major information resource for those working in the area. |