1. Endonuclease-Mediated Long PCR and Its Application to Restriction Mapping
Endonuclease-Mediated Long PCR and Its Application to Restriction Mapping
2. Molecular Diagnosis of Medical Viruses
This review discusses the applications of qualitative and quantitative real-time PCR, nested PCR, multiplex PCR, nucleotide sequence analysis of amplified products and quality assurance with nucleic acid testing (NAT) in diagnostic laboratories.
3. A PCR-based Method for Isolation of Genomic DNA Flanking a Known DNA Sequence
A PCR-based Method for Isolation of Genomic DNA Flanking a Known DNA Sequence
4. PCR Clamping
PCR Clamping
5. Universal TA Cloning
Universal TA Cloning
6. Analysis of Specific Bacteria from Environmental Samples using a Quantitative Polymerase Chain Reaction
Analysis of Specific Bacteria from Environmental Samples using a Quantitative Polymerase Chain Reaction
7. DNA Splicing by Directed Ligation (SDL)
DNA Splicing by Directed Ligation (SDL)
8. Gene Amplification from Cryopreserved Arabidopsis thaliana Shoot Tips
This protocol will help researchers to pursue faurther research in the field of molecular genetics of plant cryostress. Interesting genes identified via these process can be cloned and plants can be transfomred for the purpose of trait enhancement and modification.