Spotted microarrays

DNA oligonucleotides are spotted onto the array. The main feature of oligonucleotide microarrays is that each gene is normally represented by more than one probe.

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Spotted microarrays

In spotted microarrays technology, oligonucleotides, cDNA or small fragments of PCR products corresponding to specific genes are spotted on the chip. A robot spotter normally does the process and one or more probes can be used for each gene.
Contrary to oligonucleotide arrays, spotted arrays are "customizable"; the user can choose the probes to be spotted according to specific experimental needs. These kinds of arrays are usually hybridized with labeled cDNA and allow double channel hybridization experiments: two cDNA samples can be labeled with two different fluorophores (e.g. Cy3 and Cy5); these are mixed together and hybridized to the same chip. After washing, the chip is scanned at different wavelengths, thus allowing the measurement of the two samples in one run. Microarrays Resources Stanford Microarray Database (SMD)
Stores raw and normalized data from microarray experiments, as well as their corresponding image files. In addition, SMD provides interfaces for data retrieval, analysis and visualization.
Microarray SOURCE
SOURCE is specifically designed to facilitate the analysis of large sets of data that biologists can now produce using genome-scale experimental approaches.
Microarray GeneCards
GeneCards integrates a subset of the information stored in major data sources dealing with human genes and their products (with a major focus on medical aspects). GeneCards allows the access to expression data extracted from ESTs libraries analysis
Gene Expression Omnibus
A public repository for a wide range of high-throughput experimental data. These data include single and dual channel microarray-based experiments measuring mRNA, genomic DNA and protein abundance, as well as non-array techniques such as serial analysis of gene expression (SAGE), and mass spectrometry proteomic data.
The Microarray Gene Expression Data (MGED) Society
An international organization of biologists, computer scientists, and data analysts that aims to facilitate the sharing of microarray data generated by functional genomics and proteomics experiments.
ArrayExpress
ArrayExpress is a public repository for microarray data. Besides the browsing and querying of the data it offers the possibility to export the data for further analysis with the EBI's free analysis software, Expression profiler