Peptide Nucleic Acids: Protocols and Applications (Second Edition)
Publisher: Horizon Bioscience
Editor: Peter E. Nielsen The Panum Institute, Copenhagen
Publication date: January 2004
ISBN-10: 0-9545232-4-5
ISBN-13: 978-0-9545232-4-4
Price: GB £111 or US $222 (hardback).
Pages: viii + 318
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This second edition has been extensively revised, updated, and enlarged to include new material covering the most recent topics and applications in this fast-moving field. The book contains state-of-the-art protocols and applications on all aspects of Peptide Nucleic Acids (PNA) and in addition describes the theory and history of PNA. Concepts are explained clearly and in practical terms and each chapter contains concise background information. Written by leading experts in the field, the book is an invaluable contribution to this novel and exciting area. An important reference text and laboratory manual for all molecular biologists.
Key Features:
• Completely up-to-date
• Extensively revised
• Written by leading experts in the field
• Illustrated throughout
• Comprehensive reference sections
• Covers PNA theory and technology
• Focus on current topics and applications
Reviews:
"This is an extensively revised and updated edition of an important reference text and laboratory manual for molecular biologists ... highly recommended" from E-Streams (May 2005) Vol 8 No 5.
"This book is an excellent guide to PNAs and I would recommend it highly for anyone wanting an up to date and detailed text on this technology." from Australian J. Med. Sci. (May 2005) 26(2): 69-70..
Chapter List:
Chapter 1. An Introduction to PNA
Peter E. Nielsen and Michael Egholm
Chapter 2. PNA Synthesis by Boc Chemistry
Troels Koch
Chapter 3. Synthesis of PNA Oligomers by Fmoc Chemistry
Ralph Casale, Ivar S. Jensen, and Michael Egholm
Chapter 4. Thermodynamics of PNA Interactions
Tommi Ratilainen, Anders Holmén and Bengt Nordén
Chapter 5. Peptide Nucleic Acid Targeting of Double-Stranded DNA
Thomas Bentin, H. Jakob Larsen, and Peter E. Nielsen
Chapter 6. PNA Beacons and Fluoroprobes
Bhaskar Datta, Miaomiao Wang, and Bruce A. Armitage
Chapter 7. Vector Tagging Via PNA
Alex G. Rebuffat, Alessio G. Bernasconi and Brigitte M. Frey
Chapter 8. PCR Clamping
Henrik Ørum
Chapter 9. PNA Directed Genome Rare Cutting: New Developments
Maxim D. Frank-Kamenetskii and Vadim V. Demidov
Chapter 10. PNA Openers for Duplex DNA: Basic facts, Fine Tuning and Emerging Applications
Vadim V. Demidov
Chapter 11. Combined Use of PNA and DNA for Fluorescence
In Situ Hybridization (FISH)
Kirsten Vang Nielsen, Sven Müller, Tim Svenstrup Poulsen, Susanne Gabs, Andreas Schønau
Chapter 12. Production of PNA-Arrays for Nucleic Acid Detection
Anette Jacob, Ole Brandt, Simone Würtz, Achim Stephan, Martina Schnölzer and Jörg D. Hoheisel
Chapter 13. PNA Antisense in Eukaryotic Systems
David R. Corey
Chapter 14. Antisense Effects in Escherichia coli
Liam Good, Rikard Dryselius and Peter E. Nielsen
Appendix
• Guidelines for Sequence Design of PNA Oligomers
• Handling and Storage
• Buffers and Ionic Strength
• Quantification
• Melting Experiments
• Trouble Shooting
• Nomenclature
(EAN: 9780954523244)
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