Gene Cloning and Analysis: Current Innovations
Publisher: Horizon Scientific Press
Editor: Brian C. Schaefer Howard Hughes Medical Institute, Denver, Colorado, USA
Publication date: February 1997
ISBN-10: 1-898486-06-9 (pbk), 1-898486-11-5 (hbk)
ISBN-13: 978-1-898486-06-0 (pbk), 978-1-898486-11-4 (hbk)
Price: GB £34 or US $68 (pbk), GB £69 or US $138 (hbk)
Pages: 214
Chapter abstracts . Book reviews . How to Order
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This volume focuses on newly emerging technologies that facilitate the isolation and characterization of genes. The detailed protocols will be useful to the seasoned professional and easily understood by the novice. The vast majority of methods are applicable to any biological system, ranging from bacteria and fungi to plants, yeast, and higher eukaryotes. The protocols are accompanied by helpful notes and troubleshooting tips to ensure their successful execution. Additionally each chapter includes concise background information and frequently includes a review of similar methods followed by an assessment of future innovations.
For experienced molecular biologists, the book will provide invaluable information about the latest innovations in rapidly developing areas and expert troubleshooting advice to overcome common problems with historically difficult techniques. Not only useful, but an indispensable laboratory resource. Topics covered include:
• yeast two-hybrid system
• PNA for nucleic acid capture
• PCR for isolation of flanking DNA
• RNA ligase-mediated RACE
• site-directed mutagenesis
• PCR-ligation-PCR-mutagenesis
• universal TA cloning
• long PCR
• SSCP analysis
• cloning by protein-ligand interaction
• gene expression fingerprinting
• and much, much more
Chapter List
• Adjustment of Parameters in the Yeast Two-Hybrid System: Criteria for Detecting Physiologically Significant Protein-Protein Interactions. Erica A. Golemis, Ilya Serebriiskii and Susan F. Law.
• pJUFO: A Phage Surface Display System for Cloning Genes Based on Protein-Ligand Interaction. Reto Crameri.
• Restriction Endonuclease-Based Gene Expression Fingerprinting. N.B.Ivanova and A.V.Belyavsky.
• PNA Used for Specific Capture of Nucleic Acids. Martin B. Borre, Michael Naesby and Henrik ¯rum.
• A PCR-Based Method for Isolation of Genomic DNA Flanking a Known DNA Sequence. Catherine A. Boulter and Dipa Natarajan.
• Affinity Selection of Eukaryotic mRNAs and full-length cDNAS by a CAP Retention Procedure (CAPTURE). Jerry Pelletier.
• RNA Ligase-Mediated RACE: an Effective Method for the Cloning of Full-Length cDNA Ends. Brian C. Schaefer.
• Combinatorial Scanning Site-Directed Mutagenesis. Jean Chatellier and Thierry Vernet.
• A Rapid Method for Creating Gene Mutations and Fusions using PCR-Ligation-PCR Mutagenesis. Stuart Alvaro Ali and Alexander Steinkasserer.
• Double Replacement Gene Targeting in Embryonic Stem Cells for the Introduction of Subtle Alterations into Endogenous Mouse Genes. David W Melton.
• Universal TA Cloning. Ming-Yi Zhou and Celso E Gomez-Sanchez.
• Long PCR: Rapid Restriction Mapping and Selective Suppression by Restriction Digestion. Chengtao Her and Richard M. Weinshilboum.
• High-Resolution Silver Staining-Based PCR-Single Strand Conformation Polymorphism (SSCP) Analysis. Langxing Pan and Huaizheng Peng.
(EAN: 9781898486060, 9781898486114)
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