Intracellular Ribozyme Applications: Principles and Protocols
Publisher: Horizon Scientific Press
Editors: John J. Rossi and Larry Couture Duarte, California
Publication date: July 1999
ISBN-10: 1-898486-17-4 (hbk)
ISBN-13: 978-1-898486-17-6 (hbk)
Price: GB £95 or US $190
Pages: x + 289
Chapter abstracts . Book reviews . How to Order
Foreword by Thomas R. Cech, Howard Hughes Medical Institute, Boulder, Colorado.
In this definitive guide to intracellular ribozyme applications, researchers from both industry and academia review the most recent pharmaceutical, therapeutic, and biotechnological applications of ribozymes. Leading scientists from around the world present the latest principles on the application of ribozyme technology to the inhibition of gene expression in living cells. The authors are experts in the field and describe, in detail, protocols for monitoring ribozyme activity, for expressing ribozymes from DNA vectors, for delivering ribozymes to cells, and for in vitro selection of improved ribozymes. The book provides a forward-looking view of ribozyme technology for alteration of gene expression in plants and in treatment of retroviral and genetic diseases in man.
Key Features:
• Expert authors |
• Up-to-date |
• Detailed protocols |
• Definitive reviews |
• Comprehensive index |
• Extensive bibliographies |
Key Topics:
• Selection of Ribozymes |
• Kinetics |
• Ribozyme Expression |
• Delivery of Ribozymes |
• Detection |
• Pharmackodynamics |
• Applications |
• Therapeutic Potential |
• Inhibition of HIV Replication |
Chapter List
1. In Vitro Selection of Self-cleaving Ribozymes and Deoxyribozymes
Ronald R. Breaker
2. Developing Ribozymes for Therapeutic Applications Through In Vitro Evolution
Roshan M. Kumar and Gerald F. Joyce
3. Approaches for In Vivo Selection of Intracellurary Active Ribozymes
Makiko Hamada, Hiroaki Kawasaki, Satoshi Fujita, and Kazunari Taira
4. Rapid Determination and Quantitation of the Accessibility to Native RNAs by Ribozymes in Cell Extracts
Michaela Scherr and John J. Rossi
5. In vitro Kinetic Evaluation of Hammerhead Ribozymes
Anton P. McCaffrey and Olke C. Uhlenbeck
6. Cytoplasmic Expression of Ribozyme Using a T7 Autogene System
Yuefeng Xie, Yunsheng Li, Kelly Walker, Qin Zhu, Wenlian Xu, Thomas E. Wagner, and Xiaozhuo Chen
7. Expressing Active Ribozymes in Cells
Dmitry Samarsky, Gerardo Ferbeyre, Edouard Bertrand
8. The Use of Small RNAs as Expression Cassettes for the Delivery of Therapeutic RNAs
Cynthia P. Paul, Paul D. Good, and David R. Engelke
9. Targeted Ribozymes to Study Gene Function in Drosophila
Jack Jiagang Zhao, Carlos E.Vanario-Alonso and Leslie Pick
10. Screening Promoters for Optimal Expression of Ribozymes.
Richard Tritz, Mark Leavitt and Jack Barber
11. Lipid-Based Carriers for the Delivery of Synthetic Hammerhead Ribozymes
Mark A. Reynolds
12. Retroviral Vector Mediated Intracellular Delivery of Ribozyme Genes
Ingrid Bahner
13. Adeno-associated Virus Vectors for Transduction of Genes Encoding Ribozymes
Saswati Chatterjee and K.K. Wong, Jr
14. RNA Analysis by Terminal Transferase-Dependent PCR
Hsiu-Hua Chen, Daniela Castanotto, John J. Rossi and Arthur D. Riggs
15. Ribozyme Pharmacokinetic Screening for Predicting Pharmacodynamic Dosing Regimens
Tom J. Parry, Pamela A. Pavco, Jennifer A. Sandberg
16. Ribozymes for the Treatment of Restenosis following PTCA
Joan Robbins, Aaron Frimerman, Neal Eigler, Frank Litvack, Jack Barber
17. The Use of Ribozyme Gene Therapy for the Inhibition of HIV Replication and its Pathogenic Sequelae
Justin E. Rigden, Julie A. Ely, Janet L. Macpherson, Wayne L. Gerlach, Lun-Quan Sun and Geoff P. Symonds
18. Ribozyme Genes Protecting Transgenic Melon Plants Against Potyviruses
Eric Huttner , William Tucker, Agnès Vermeulen, Frédéric Ignart, Brett Sawyer, and Robert Birch
(EAN: 9781898486176)
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