Microbiology news and views
J. Mol. Micro. Biotechnol. 3: 179-184
Enterococcus faecalis Multi-Drug Resistance Transporters: Application for Antibiotic Discovery
Deborah vR. Davis, James B. McAlpine, Christopher J. Pazoles, M. Kelly Talbot, Elisabeth A. Alder, Abbie C. White, Brandie
M. Jonas, Barbara E. Murray, George M. Weinstock, and Bruce L. Rogers
Using bioinformatics approaches, 34 potential multi-drug resistance (MDR) transporter sequences
representing 4 different transporter families were identified in the unannotated
Enterococcus faecalis database (TIGR). A functional genomics campaign generating single-gene insertional disruptions revealed several genes
whose absence confers significant hypersensitivities to known antimicrobials. We constructed specific
strains, disrupted in a variety of previously unpublished, putative MDR transporter genes, as tools to improve
the success of whole-cell antimicrobial screening and discovery. Each of the potential transporters was
inactivated at the gene level and then phenotypically characterized, both with single disruption mutants and with
2-gene mutants built upon a D norA deleted strain background.
Full article [pdf]
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