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J. Mol. Micro. Biotechnol. 3: 319-324
Comparative Approach to Analysis of Regulation in Complete Genomes: Multidrug Resistance Systems in Gamma-Proteobacteria
Dmitry A. Rodionov, Mikhail S. Gelfand, Andrey A. Mironov, and Alexandra B. Rakhmaninova
Comparative approach is a powerful tool for analysis of gene regulation in bacterial genomes. Here we apply
it to analysis of regulation of the multidrug resistance transport (MDRT) systems in
enterobacteria Escherichia coli, Salmonella
typhi, Klebsiella pneumoniae and Yersinia
pestis. Comparison of nucleotide sequences
upstream of MDRT genes was performed in order to predict new regulatory sites (operators) and identify candidate
regulons. Since the regulatory sites diverge slower than the non-coding regions in general, they are visible as
strongly conserved islands. This analysis resulted in description of a regulatory network for known and
hypothetical MDRT systems and porins. New candidate members of the MarA regulon were detected. Putative binding
sites for EmrR and AcrR were suggested. A new hypothetical MarX regulon was described that includes some
multidrug transporters and porins.
Full article [pdf]
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