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J. Mol. Micro. Biotechnol. 3: 461-465
Antibacterial Activity of 4,5-Dihydroxy-2-cyclopentan-1-one (DHCP) and Cloning of a Gene Conferring DHCP Resistance in Escherichia coli
Sangita Phadtare, Kunitoshi Yamanaka, Ikunoshin Kato and Masayori Inouye
In the present study we report that 4,5-dihydroxy-2-cyclopentan-1-one (DHCP), which is derived from heattreatment of uronic acid or its derivatives, has antibacterial activity against Escherichia coli. The compound causes complete growth inhibition at 350 mM concentration. We have cloned a gene from E. coli, which confers DHCP resistance when present in multicopy. The putative protein encoded by this gene (dep - DHCP efflux protein) is a transmembrane efflux protein with a high homology to other antibioticefflux proteins including those for chloramphenicol, bicyclomycin and tetracycline. However, the Dep protein does not confer cross-resistance to any of the antibiotics tested.
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