JMMB Abstract

J. Mol. Micro. Biotechnol. 4:551-554

Approaches to Library Screening

Tessa N. Campbell and Francis Y.M. Choy

Fuelled by the drive to complete the Human Genome Project, many laboratories have developed new methods of screening clone libraries. From PCR-based strategies to pooling schemes and increased automation, the tedious task of library screening has become less labourintensive and more cost-efficient. Currently, two main screening methods dominate: hybridization and polymerase chain reaction (PCR). In the following article, we present a brief overview of hybridization and PCR-based screening of yeast and bacterial libraries. Multi-faceted approaches combining different techniques, as well as less frequently employed methods such as fingerprinting are also described.-

Full article [pdf]

Approaches to Library Screening

Tessa N. Campbell and Francis Y.M. Choy

Recommended Reading