Isolation of DNA from buccal swabs for the analysis of genomic DNA is becoming common for research and forensic purposes. Here, we demonstrate that urine provides a good alternative for obtaining small amounts of PCR-ready DNA.
We developed a rapid and safe method for extracting DNA from both sources using components of Qiagen's PCR cleanup kitTM. PCR amplification on the isolated DNA gave consistent and good results. In addition, conditions for the storage of unprocessed urine and buccal swabs are given. One isolation takes less than 10 minutes and is enough for at least ten PCR reactions.
