PCR Theory

Lab-on-a-Chip Technology (Vol. 2): Biomolecular Separation and Analysis

Edited by: Keith E. Herold and Avraham Rasooly
Applications in the biomedical and life sciences: biomolecule separation, electrophoresis, chromatography, protein and cell separation, genetic and transcriptome analysis, PCR, cell viability analysis and microorganism capturing.

"a comprehensive and felicitous compendium" (Drug Research) read more ...

The Theory of Legacy PCR

By legacy PCR we mean the original, traditional PCR performed in a tube. When the reaction is complete the products of the reaction (the amplified DNA fragments) are analysed and visualised by gel electrophoresis. For more detailed information see PCR Basic Theory ...

Real-Time PCR theory The Theory of Real-Time PCR

Real-time PCR is achieved by the use of fluorescent dyes that can be measured by a detection system. This also permits the quantitation of the DNA. For more detailed information about real-time PCR please see Real-Time PCR Theory ...

The Principle of PCR

PCR consists of an exponential amplification of a DNA fragment, and its principle is based on the mechanism of DNA replication in vivo: dsDNA is denatured to ssDNA, duplicated, and this process is repeated along the reaction according to a specific formula. For a more detailed treatment of the principle of PCR please see The Principle of PCR ...

Recommended Reading

	Real-Time PCR: Advanced Technologies and Applications Edited by: Nick A. Saunders and Martin A. Lee Provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications and supplies detailed technical insights into the underlying principles, methods and practice of real-time PCR. read more ...
	Real-Time PCR in Food Science: Current Technology and Applications Edited by: David Rodríguez-Lázaro An indispensable manual on real-time PCR for scientists in the food industry and for anyone involved in the detection of foodborne pathogens. read more ...
	Quantitative Real-time PCR in Applied Microbiology Edited by: Martin Filion Aimed specifically at microbiologists, this volume describes and explains the most important aspects of current real-time quantitative PCR (qPCR) strategies, instrumentation and software. "useful book ... filled with valuable information" (Doodys) read more ...
	PCR Troubleshooting and Optimization: The Essential Guide Edited by: Suzanne Kennedy and Nick Oswald Control, optimize and troubleshoot PCR, reverse transcriptase PCR, real-time PCR and quantitative PCR. An essential book. "an essential book ... a valuable tool to all those interested in PCR" (Doodys); "an essential guide" Aus. J. Med. Sci. read more ...
	Lab-on-a-Chip Technology (Vol. 2): Biomolecular Separation and Analysis Edited by: Keith E. Herold and Avraham Rasooly Applications in the biomedical and life sciences: biomolecule separation, electrophoresis, chromatography, protein and cell separation, genetic and transcriptome analysis, PCR, cell viability analysis and microorganism capturing. "a comprehensive and felicitous compendium" (Drug Research) read more ...