Temperature Specifications of Real-time PCR instruments and qPCR machines
from Julie Logan, Kirstin Edwards and Nick Saunders in Real-Time PCR: Current Technology and Applications | Edited by: Keith E. Herold and Avraham Rasooly "a comprehensive and felicitous compendium" (Drug Research) read more ...Applications in the biomedical and life sciences: biomolecule separation, electrophoresis, chromatography, protein and cell separation, genetic and transcriptome analysis, PCR, cell viability analysis and microorganism capturing. |
|
Platform |
Max. heating/ cooling rate (°C/sec) |
Temperature Accuracy (°C) |
Temperature Uniformity (°C) |
|
ABI 7900HT Fast |
3.0/3.0 |
+/- 0.25 |
+/- 0.5 |
|
ABI 7300 |
1.1/1.1 |
+/- 0.25 |
+/- 0.5 |
|
ABI 7500 |
0.8/1.6 |
+/- 0.25 |
+/- 0.5 |
|
ABI 7500 Fast |
3.5/3.5 |
+/- 0.25 |
+/- 0.5 |
|
ABI StepOne |
2.2/2.2 |
+/- 0.25 |
+/- 0.5 |
|
Mastercycler ep realplex4 |
6.0/4.5 |
+/- 0.2 |
+/- 0.3 |
|
Roche LightCycler |
20.0/20.0 |
+/- 0.4 |
+/- 0.2 |
|
Roche LightCycler 480 |
4.8 /2.5 |
+/- 0.2 |
|
|
BioRad MiniOpticon |
2.5/2.0 |
+/- 0.2 |
+/- 0.4 |
|
BioRad MyiQ |
3.3/2.0 |
+/- 0.3 |
+/- 0.4 |
|
BioRad Opticon 2 |
3.0/2.0 |
+/- 0.4 |
+/- 0.4 |
|
BioRad Chromo4 |
3.0/2.0 |
+/- 0.3 |
+/- 0.4 |
|
BioRad iQ5 |
3.3/2.0 |
+/- 0.3 |
+/- 0.4 |
|
Stratagene Mx4000 |
2.2/2.2 |
+/- 0.25 |
+/- 0.25 |
|
Stratagene Mx3000P |
2.5/2.5 |
+/- 0.25 |
+/- 0.25 |
|
Stratagene Mx3005P |
2.5/2.5 |
+/- 0.25 |
+/- 0.25 |
|
Corbett Rotor-Gene 6000 |
15.0/20.0 |
+/- 0.25 |
+/- 0.01 |
|
Cepheid SmartCycler |
10.0/2.5 |
+/- 0.5 |
+/- 0.5 |
| Edited by: Nick A. Saunders and Martin A. Lee read more ...Provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications and supplies detailed technical insights into the underlying principles, methods and practice of real-time PCR. |
| Edited by: David Rodríguez-Lázaro read more ...An indispensable manual on real-time PCR for scientists in the food industry and for anyone involved in the detection of foodborne pathogens. |
| Edited by: Martin Filion "useful book ... filled with valuable information" (Doodys) read more ...Aimed specifically at microbiologists, this volume describes and explains the most important aspects of current real-time quantitative PCR (qPCR) strategies, instrumentation and software. |
 | Edited by: Suzanne Kennedy and Nick Oswald "an essential book ... a valuable tool to all those interested in PCR" (Doodys); "an essential guide" Aus. J. Med. Sci. read more ...Control, optimize and troubleshoot PCR, reverse transcriptase PCR, real-time PCR and quantitative PCR. An essential book. |
| Edited by: Keith E. Herold and Avraham Rasooly "a comprehensive and felicitous compendium" (Drug Research) read more ...Applications in the biomedical and life sciences: biomolecule separation, electrophoresis, chromatography, protein and cell separation, genetic and transcriptome analysis, PCR, cell viability analysis and microorganism capturing. |