Featured books:
What is PCR?
One of the most common search phrases is What is PCR? | Edited by: Keith E. Herold and Avraham Rasooly "a comprehensive and felicitous compendium" (Drug Research) read more ...Applications in the biomedical and life sciences: biomolecule separation, electrophoresis, chromatography, protein and cell separation, genetic and transcriptome analysis, PCR, cell viability analysis and microorganism capturing. |
What is PCR
PCR is an abbreviation for Polymerase Chain Reaction. PCR is a process used to makes copies of a piece of DNA. PCR consists of three steps: Denaturation, annealing, and extension. Each PCR stage doubles the number of DNA molecules. The process is continued for many cycles to generate a huge number of copies. The process is performed on a PCR cycler or PCR machine. The PCR cycler heats or cools the PCR mixture at the appropriate time in order to allow denaturation, annealing, or extension. The principles of Polymerase Chain Reaction (PCR) were described for the first time in 1971. But it was in 1985, with the introduction of a thermostable DNA polymerase, that the first experimental data using this technique was published. Since then, the use of PCR has been described in hundreds of thousands of scientific publications. The PCR technique has been applied in many different scientific areas due to its versatility, specificity and sensitivity.- PCR Theory An introduction to the theory of the polymerase chain reaction
- What is PCR? A definition of PCR
- Legacy PCR Theory The theory of traditional, legacy PCR
- Real-time PCR Theory The theory of real-time PCR
- The Principle of PCR Detailed PCR principles with equations for calculating the final amount of DNA and the final number of copies of the target sequence
- Multiplex PCR
- Real-Time PCR Real-time PCR allows monitoring of the progress of a PCR reaction while the reaction is actually in progress. The technology also permits the quantitation of DNA products
- Theory of Real-Time PCR An introduction to the theory of real-time PCR
- Real-Time PCR Machines
- Features of Real-Time PCR Machines A comparison of different, commercially-available, machines
- Temperature Specifications Temperature specifications of real-time machines
- Fluorescence Detection Real-time PCR chemistries and detection formats
- Real-Time PCR Design
- Quantification Analysis Real-time PCR Quantification Analysis
- Real-time PCR Quality Control
- Real-time PCR Parameters
- PCR Technology
- PCR Primer Design A very important aspect of PCR and real-time PCR
- PCR Protocols PCR protocols available free online
- PCR Applications A description of some common applications of PCR and real-time PCR
- PCR Troubleshooting A guide to troubleshooting and optimization of PCR methods and technology
- Publications on PCR
- PCR Book Reviews Excerpts from published reviews of current books on PCR
- PCR Reviews Free articles to download
- PCR Resources Online resources
Recommended Reading
| Edited by: Nick A. Saunders and Martin A. Lee read more ...Provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications and supplies detailed technical insights into the underlying principles, methods and practice of real-time PCR. |
| Edited by: David Rodríguez-Lázaro read more ...An indispensable manual on real-time PCR for scientists in the food industry and for anyone involved in the detection of foodborne pathogens. |
| Edited by: Martin Filion "useful book ... filled with valuable information" (Doodys) read more ...Aimed specifically at microbiologists, this volume describes and explains the most important aspects of current real-time quantitative PCR (qPCR) strategies, instrumentation and software. |
 | Edited by: Suzanne Kennedy and Nick Oswald "an essential book ... a valuable tool to all those interested in PCR" (Doodys); "an essential guide" Aus. J. Med. Sci. read more ...Control, optimize and troubleshoot PCR, reverse transcriptase PCR, real-time PCR and quantitative PCR. An essential book. |
| Edited by: Keith E. Herold and Avraham Rasooly "a comprehensive and felicitous compendium" (Drug Research) read more ...Applications in the biomedical and life sciences: biomolecule separation, electrophoresis, chromatography, protein and cell separation, genetic and transcriptome analysis, PCR, cell viability analysis and microorganism capturing. |